SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism inC. eleganscentriole assembly

Structures of SAS-6 suggest its organization in centrioles.

Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombin...

Structure of the SAS-6 cartwheel hub from Leishmania major

Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Lei...

The structure of the GemC1 coiled coil and its interaction with the Geminin family of coiled-coil proteins

GemC1, together with Idas and Geminin, an important regulator of DNA-replication licensing and differentiation decisions, constitute a superfamily sharing a homologous central coiled-coil domain. To better understand this family of proteins, the crystal structure of a GemC1 coiled-coil domain variant engineered for better solubility was determined to 2.2 Å resolution. GemC1 shows a less typical...

Confidence intervals for the interaction contrast ratio: A simple solution with SAS PROC NLMIXED and SAS PROC NLP

Indexing in the SAS System , Version 6 ®

The purpose of this article is to explain indexing in the SAS System. Questions and answers are provided to help ® you understand what an index is and how one works, when and how to create an index, when SAS uses an index, how an index is maintained, and what you can do to enhance the performance of an index. Content The following topics are discussed: • Introduction • Understanding the Index f...